supplemented with 20 mM HEPES (pH 7.3; Gibco 15630106). Dulbecco’s phosphate-buffered saline (DPBS) was from Gibco (14040133). The 5 mM pH = 7.3 phosphate buffer was prepared by diluting a 0.1 M ...
In summary, these BLI data demonstrate that B. subtilis MinD is capable of binding membrane in both monomeric and dimeric form, while the presence of ATP in the binding pocket appears to affect both ...
The obtained cells were finally resuspended in RPMI 1640 medium containing 5% FCS, 1% penicillin and streptomycin (all from PAA Laboratories), 2 mM L-glutamine and 25 mM HEPES (Sigma-Aldrich). 2.4.2 ...
Characterization of hyperpolarization-activated cyclic nucleotide-gated channels in oligodendrocytes
Sigma Aldrich), 5 mL SATO (see below), 5 mL glutamine (Invitrogen), and 10 mL of B27 Neurobrew without Vitamin A (Invitrogen). For internal solution (in mM): 130 KCl, 10 NaCl, 0.5 MgCl 2, 1 EGTA, 5 ...
How the hydrolytic activity of mitochondrial ATP synthase is modulated during brown adipose tissue (BAT) thermogenesis ...
To address the unmet need for a cure for RTT, the therapeutic potential of adult Neural Precursor Cells (NPCs) was ...
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